ea0012p34 | Cytokines, growth factors, growth and development | SFE2006
Burns CJ
, Robinson CJ
The objective of this study is to investigate the use of qRT-PCR as an early-stage bioassay to quantify biological activity. The action of VEGF165 on human umbilical vein endothelial cells (HUVECs) was selected as a model since we have previously studied dose-dependence and time courses for several late-stage responses for this system (production of IL-6, IL-8 and tissue factor, cellular proliferation and survival). Assay of VEGF activity and inhibitors underpins a ...